y小鼠心脏组织IRE1α和XBP1表达的影响> 黄芪注射液联合葛根素注射液对2型糖尿病动物K(1)

http://www.100md.com 2017年6月15日 《世界中医药》 201712

     摘要 目的：探讨黄芪注射液联合葛根素注射液对2型糖尿病动物KKAy小鼠的心脏保护作用机制，为中医药治疗糖尿病心肌病提供实验依据。方法：将造模成功的KKAy小鼠按血糖随机分为模型组和观察组(黄芪注射液联合葛根素注射液)，同周龄雄性C57BL/6J小鼠作为正常对照组，并于20周龄、24周龄、28周龄分别处死小鼠。荧光定量PCR测小鼠心脏组织中IRE1α、XBP1U、XBP1S的表达，蛋白免疫印迹检测小鼠心脏组织中IRE1α、XBP1蛋白的表达。结果：模型组小鼠20、24、28周龄心脏组织IRE1α的 mRNA和蛋白表达比正常组明显增加(P<0.01)，观察组20周龄IRE1α蛋白表达较正常组增加(P<0.01)，但观察组各周龄IRE1αmRNA和蛋白表达均低于模型组(P<0.01或P<0.05)；模型组小鼠各周龄XBP1S mRNA和XBP1蛋白表达、28周龄XBP1U的mRNA表达比正常组增加(P<0.01或P<0.05)，观察组各周龄XBP1蛋白表达和20、24周龄的XBP1S mRNA表达较模型组降低，而28周龄XBP1S和XBP1U的mRNA表达均分别比模型组和正常组增加(P<0.05)。结论：黄芪注射液联合葛根素注射液可在一定程度上抑制2型糖尿病动物KKAy小鼠心脏组织中内质网应激信号分子IRE1α和XBP1的表达，减轻内质网应激，保护2型糖尿病KKAy小鼠的心脏功能。
, 百拇医药
    关键词 黄芪注射液；葛根素注射液；糖尿病心肌病；内质网应激

    Abstract Objective：To investigate the cardioprotective mechanism of Huangqi injection combined with puerarin injection on KKAy mice with diabetic cardiomyopathy， providing experimental evidence for the treatment of diabetic cardiomyopathy using traditional Chinese medicine. Methods KKAy mice were randomly divided into model group and treatment group (Huangqi injection combined with puerarin injection) according to blood glucose. The male C57BL/6J mice with same age were used as normal control group. The mice were sacrificed at 20， 24 and 28 weeks old， respectively. qRT-PCR was used for detecting the mRNA expression of IRE1α， XBP1U and XBP1S and Western blotting was used for assaying the expression of IRE1α and XBP1. Results：The mRNA and protein expression of IRE1α in the model group increased at 20， 24 and 28 weeks， while the increase of mRNA and protein expression of IRE1α reduced in the treatment group at 20， 24 and 28 weeks. The difference was statistically significant (P<0.05). The mRNA and protein expression of XBP1S at 20， 24 and 28 weeks and the mRNA expression of XBP1U at 28 weeks was increased in the model group； For the treatment group， the mRNA expression of XBP1S at 20 and 40 weeks and protein expression of XBP1 was down-regulated， whereas the expression of XBP1S and XBP1U mRNA increased. The difference was statistically significant (P<0.05). Conclusion：Huangqi injection combined with puerarin injection could inhibit the expression of IRE1α and XBP1， two stress signaling molecules of endoplasmic reticulum in the heart tissue of KKAy mice， and reduce the endoplasmic reticulum stress and exert cardioprotective effect on KKAy mice with type 2 diabetes.

    Key Words Huangqi injection； Puerarin injection； Diabetic cardiomyopathy； Endoplasmic reticulum stress

    中圖分类号：R228文献标识码：Adoi：10.3969/j.issn.1673-7202.2017.12.048, 百拇医药(张淑静 王谦 李姝玉 赵福建 王岩飞 高誉珊 李奕萱 张达)

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